![]() ![]() Food additive Īs a food additive, ammonium sulfate is considered generally recognized as safe (GRAS) by the U.S. Selective precipitation with ammonium sulfate, opposite to the usual precipitation technique which uses acetic acid, does not interfere with the determination of volatile fatty acids. In the analysis of rubber lattices, volatile fatty acids are analyzed by precipitating rubber with a 35% ammonium sulfate solution, which leaves a clear liquid from which volatile fatty acids are regenerated with sulfuric acid and then distilled with steam. Ammonium sulfate precipitation provides a convenient and simple means to fractionate complex protein mixtures. Precipitation by ammonium sulfate is a result of a reduction in solubility rather than protein denaturation, thus the precipitated protein can be solubilized through the use of standard buffers. A high salt concentration, which can be achieved by adding or increasing the concentration of ammonium sulfate in a solution, enables protein separation based on a decrease in protein solubility this separation may be achieved by centrifugation. As such, although high concentrations are needed for the method to work adding an abundance of the salt, over 100%, can also oversaturate the solution, therefore, contaminating the nonpolar precipitate with salt precipitate. The percentage of the salt used is in comparison to the maximal concentration of the salt in the mixture can dissolve. This method is called salting out and necessitates the use of high salt concentrations that can reliably dissolve in the aqueous mixture. The significance of this substance in the purification of compounds stems from its ability to become more so hydrated compared to relatively more nonpolar molecules and so the desirable nonpolar molecules coalesce and precipitate out of the solution in a concentrated form. Due to the high dielectric constant of water, the dissociated salt ions being cationic ammonium and anionic sulfate are readily solvated within hydration shells of water molecules. Ammonium sulfate is extremely soluble in water due to its ionic nature, therefore it can "salt out" proteins by precipitation. As the ionic strength of a solution increases, the solubility of proteins in that solution decreases. It is particularly effective as an adjuvant for 2,4-D (amine), glyphosate, and glufosinate herbicides.Īmmonium sulfate precipitation is a common method for protein purification by precipitation. There, it functions to bind iron and calcium cations that are present in both well water and plant cells. It is also used as an agricultural spray adjuvant for water-soluble insecticides, herbicides, and fungicides. The main disadvantage to the use of ammonium sulfate is its low nitrogen content relative to ammonium nitrate, which elevates transportation costs. In the soil the ammonium ion is released and forms a small amount of acid, lowering the pH balance of the soil, while contributing essential nitrogen for plant growth. The primary use of ammonium sulfate is as a fertilizer for alkaline soils. ![]() The most common use is as a soil fertilizer. ![]() Ammonium sulfate (American English and international scientific usage ammonium sulphate in British English) (NH 4) 2SO 4, is an inorganic salt with a number of commercial uses. ![]()
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